Effect of knockout of ALKBH5 on gene expression and m6A modifications during IRI in mice. Effect of knockout of ALKBH5 on gene expression and m6A modifications during IRI in mice

To identify the direct m6A demethylation targets of ALKBH5 at the onset of renal IRI, we performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) using RNA isolated from IRI mouse kidney of WT and KO mice 24h after I/R (WT n=3 vs. KO n=3) Overall design: WT group (n=3) VS KO group (n=3)