Genome-wide transcriptome analysis of liver sinusoidal endothelial cells in the pathogenesis of NASH

We isolated liver sinusoidal endothelial cells (LSEC) from mice fed control chow (Chow) or the nonalcoholic steatohepatitis (NASH)-inducing high fat, fructose and cholesterol (FFC) diet, and subjected the cells to bulk RNA-seq. Out of 13993 transcripts detected, the adhesion molecule Vcam1 was significantly upregulated in LSEC from FFC-fed mice compared to Chow-fed mice (Log2FC: 0.925, p: 3.6x10E-18), whereas the upregulation of another adhesion molecule Icam1 (Log2FC: 0.416, p: 0.014) was not as striking as Vcam1. Overall design: Liver sinusoidial endothelial cells from NASH and control mice were subjected to bulk RNA-seq analysis.