Gene expression of zebrafish larvae upon treating with lalistat. Gene expression of zebrafish larvae upon treating with lalistat

To test if HIF pathway is regulated by lipids in zebrafish larvae, we have employed whole genome microarray expression profiling as a discovery platform to identify induced genes upon lalistat treatment. Lalistat is a specific inhibitor for lysosomal acid lipase (LAL), which is required for break down triglyceride or cholesterol ester to release free fatty acids. Overall design: Zebrafish larvae (5dpf) were treated with vehicle control DMSO or lalistat (10uM) for 2hr. Each RNA sample were extracted from pooled 4 larvae. Two biological replicates (2 clutches from different pairs of WT zebrafish mating) were conducted.