Impact on erythroid progenitor type on erythroid differntiation

We sought to compare mature iRBCs derived from WT or SCA hiPSCs to characterize observable disease phenotypes in culture. We performed bulk mRNA sequencing on PB cRBCs, WT iRBCs and SCA iRBCs. RNA-seq was performed to determine the genome-wide changes in expression in peripheral blood derived myelo-erythroid progenitors versus wild type and sickle cell iPS progentiors at different stages of erythropoiesis. Global transcriptomic analyses revealed that SCA iRBCs had elevated expression of hypoxia-responsive genes and decreased expression of metabolic pathways relating to lipid metabolism.