Comprehensive analysis of H3K9me2 oocytes, together with DNA methylation and expression analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos. Comprehensive analysis of H3K9me2 oocytes, together with DNA methylation and expression analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos

We report the first genome-wide landscape of H3K9me2 ChIP-seq profile in mouse oocytes. We also performed whole-genome bisulfite sequencing and RNA-seq analysis of G9a conditional KO oocytes and maternal KO preimplantation embryos. Our findings illuminate the functional importance of G9a in preimplantation development and, in addition, pose a question on the proposed role for H3K9me2 in protection of the maternal genome from active CG demethylation. Overall design: ChIP-seq H3K9me2 profile was generated from wildtype fully-grown oocytes (FGOs). Whole-genome bisulfite sequencing anlaysis was performed on G9a conditional KO oocytes (growing oocytes, FGOs and MII oocytes) and maternal KO preimplantation embryos (early 2-cell embryos and blastocysts).