HDAC4 controls senescence and aging by safeguarding the epigenetic identity and ensuring the genomic integrity

Purpose: Define HDAC4 signature Outcome: HDAC4 is required for the repression of a senescence signature. HDAC4 KO promotes the expression of inflammatory genes, the derepression of ERVs and the accumulation of DNA damage. All together these signalling pathways lead to permanent cell-cycle arrest in low grade tumor cells and pre-transformation models, while they weaken the replicative potential of primary normal cells. 6 clones of SK-LMS-1 cells were created. Two controls: one expressing Cas9 as control, the other expressing Cas9 + sgRNA1. 4 HDAC4 KO clones were generated: 76 and 1231 (achieved through sgRNA1); 205 and 1254 (achieved through sgRNA2). Two biological replicates per clone were analyzed.