Langdon genome assembly and annotation

Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in agricultural research. Here, we present a high-quality chromosome-level genome assembly of Langdon using a combination of PacBio HiFi sequencing and Hi-C techniques. The total genome assembly size was 10.47 Gb, with a BUSCO score of 98.8%, QV score of 50.31, and a contig N50 value of 41.18 Mb and the longest contig reached 295.80 Mb. Repetitive elements constituted 88.13% of the assembly, and 68,342 high confidence protein-coding genes were annotated. The high-quality chromosome-level Langdon assembly can serve as a valuable reference for durum wheat, aiding gene research and comparative genomics, and leveraging synthetic hexaploid wheat's genetic resources to advance global wheat improvement.