Effects of semaglutide on the gut microbiota of high-fat diet-fed mice lacking GLP-1Rs in T cells

Gut intraepithelial lymphocytes (IELs) are one of the few immune cell populations in the body that expresses glucagon-like 1 receptors (GLP-1R). To test the potential effects of GLP-1 on the gut microbiota through the gut IEL GLP-1R, we performed 16s rRNA seq on the DNA isolated from the fecal pellet of Lck-Cre; Glp1rfl/fl mice (Glp1rTcell-/-) or controls (Glp1rTcell+/+) fed a high-fat diet (HFD) for 12 weeks followed by 1 week of HFD plus semaglutide (10 ug/kg) or vehicle treatment. Fecal pellets from a group of age-matched, sex-matched control mice were included as a chow control group. Overall design: 6 chow-fed control, 9 vehicle-treated Glp1rTcell+/+, 9 semaglutide-treated Glp1rTcell+/+, 11 vehicle-treated Glp1rTcell-/-, and 9 semaglutide-treated Glp1rTcell-/- mice. DNA isolated from fecal pellets was amplified for the V3 and V4 regions of the 16s rRNA gene, prepared into sequencing libraries, and sequenced on a MiSeq (Illumina) with a read length of 2 x 300 bp.