RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

We used high throughput sequencing to analyze the gene expression profiling of human aortic smooth muscle cells (AoSMC). By comparing the gene expression profiling of AoSMC with or without H19 knockdown, 2940 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 2790 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells. Overall design: AoSMC cells were transfected with either control siRNA or siH19. 48h after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare gene expression profiles between siCon and siH19 cells.