Km and Vmax of citrate transport in a reconstituted system.
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Km and Vmax values were measured in a reconstituted system at the times indicated. Proteoliposomes were reconstituted with the CIC as described in the Methods section. [l4C] Citrate, 0.04–0.40 mM, was added to proteoliposomes containing 10 mM citrate. The citrate/citrate exchange was stopped 1 min after the addition of the radiolabeled substrate by 20 mM 1,2,3-BTA. Km and Vmax values were calculated by linear regression.
创建时间：
2015-12-02
