Conservation analysis of residues important for the electrostatic signature of the protein.

These residues were identified as being close to low energy regions in all MD and PB profiles of Cl−. Only charged residues were considered, since they have the highest influence on the electrostatics. For each identified residue the conservation and exchange rate based on a MSA of 135 VDAC sequences are listed. For Lys200 the complete percentage does not lead to 100% since there were two gaps found at this position in the MSA. The residue numbering refers to mVDAC1.