Figure 6

Figure 6: SYN1+ nerves highlight the potential for neuroeffector-junction formation during kidney development. (A) Top panel: Immunostaining of E14.5 kidney sections for synapsin I (SYN1, magenta) shows overlap with TUBB3 (green) and association of axons with the main artery (CD31, yellow; arrowhead). Bottom panel: E16.5 kidney sections similarly show SYN1+ (magenta) and TUBB3+ (green) nerves around the vasculature (CD31, yellow; arrowhead). (B) Sections of Pod-Cretg/+;R26tdTomato/tdTomato P0 kidneys show SYN1+ axons (magenta) overlapping with TUBB3 (green) near glomeruli (tdTomato, yellow; arrowheads, top panel) and proximal tubules (LRP2, green; arrowhead, bottom panel). (D) Sections of Pod-Cretg/+;R26tdTomato/tdTomato adult kidneys show SYN1+ (magenta) and TUBB3+ (green) axons along the arterioles near glomeruli (tdTomato, yellow; arrowhead).