Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic memory impairment

In this study, we utilized snRNA-seq to explore the transcriptional changes in hippocampal neurons of drug-resistant mice with memory disorder in epilepsy, and analyzed the inter-group heterogeneity of 20 subclasses of neurons, focusing on aspects such as cell communication, gene expressions, GO and KEGG enrichment analysis, and module gene set analysis. Our data provides a comprehensive transcriptomic description of the neuronal population affected by drug-resistant epileptic memory disorders. Further studies have shown that specific neuronal subpopulations impact synaptic plasticity through the regulation of relevant target genes by specific transcription factors. Comparative gene expression analysis of SnRNA seq data between drug-resistant epileptic memory impairment and normal mouse hippocampal tissue.