Determining the effect of different adjuvants and doses on the immune response

Inbred C57BL/6 female mice (8-10 weeks old) were immunized subcutaneously according to the 8 immunization groups. Serum samples were collected weekly from 2 staggered groups and analyzed for PA-specific (Total Ig, IgG1, IgG2a, and IgG3) and neutralizing antibody titers. Also, spleen, thymus, and brachial and inguinal lymph nodes will be harvested from 1 of each group at 4 time points for histology.