Precursor lesions clonal architecture and relapse in Wilms nephroblastoma

Targeted resequencing at high depth (21 genes, 9 chromosomal regions): at least 4 FFPE samples per case and matched germline DNA: • 100 cases with detailed outcome data, including 15 cases with tumour relapse (515 samples) • 40 cases with matched pre-chemotherapy biopsies (240 samples) • 50 nephrogenic rests matched to above cases (50 samples) We expect a proportion (possibly 10%) of cases to be mutationally silent on the above studies, and propose to subsequently carry out integrated whole-genome, methylome and transcriptome studies on matched frozen tissue from these casesEGA study EGAS00001001422