The effects of testosterone on epithelial cells isolated from mouse seminal vesicles.

The male fertility depends largely on the quality of semen composed of sperm and seminal plasma. While numerous studies have focused on testicular function, research on male accessory gonads, responsible for seminal plasma synthesis, remains limited. This project investigated male infertility with a specific emphasis on seminal plasma and its synthesis mechanisms. Seminal vesicle epithelial cells were isolated from 8-week-old specific pathogen-free C57BL/6NCrl mice. The isolated cells were cultured in DMEM/F-12 containing 100 nM testosterone (Test) or 0.1% ethanol (Ctrl) and 10% fetal bovine serum for 7 days.