Metadata for Sanger Sequencing

AG groups were determined by Sanger sequencing of the internal transcribed spacer (ITS) region using the ITS1F (CTT GGT CAT TTA GAG G AAG TAA) and ITS4B (CAG GAG ACT TGT ACA CGG TCC AG) primer pair (758 bp) (Gardes and Bruns 1993). Amplicons were shipped to GENEWIZ (South Plainfield, New Jersey, USA) for purification and Sanger Sequencing. Contigs were assembled using CAP3 software (Huang and Madan 1999). Taxonomy was assigned to each OTU via the NCBI BLASTn database (https://blast.ncbi.nlm.nih.gov) with criteria as 0.0 E and nucleotide match of at least 97%. <br> Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for Basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology <strong>2</strong>:113-118. <br> Huang, X., and Madan, A. 1999. CAP3: A DNA sequence assembly program. Genome Res 9:868-877. <br> <br>