泡桐全基因组重测序数据

采集雌亲本、雄亲本和98株F1代植株的嫩叶，采用CTAB法提取基因组DNA。经过一系列操作（如DNA片段化、末端修复、添加Poly(A)尾和适配器等）后，将合格的DNA用于构建测序文库。合格的文库基于Illumina Hiseq×10平台通过双末端PE150进行测序（Illumina, San Diego, CA, USA），测序深度为10×。

Young leaves of female parent, male parent and 98 F1 progeny plants were collected, and genomic DNA was extracted via the CTAB method. After a series of procedures including DNA fragmentation, end repair, poly(A) tail addition and adapter ligation, qualified DNA samples were used to construct sequencing libraries. Qualified libraries were sequenced using paired-end PE150 strategy on the Illumina HiSeq ×10 platform (Illumina, San Diego, CA, USA), with a sequencing depth of 10×.

搜集汇总

背景与挑战

背景概述

该数据集包含泡桐全基因组重测序数据，通过采集雌雄亲本和F1代植株样本，采用CTAB法提取DNA并在Illumina平台上进行测序，生成了高质量基因组数据。数据用于支持泡桐高密度遗传图谱构建和重要性状QTL定位研究，属于林学领域的限制共享科学数据。