Microsatellite data of bank voles and <em>Ixodes ricinus</em> plus TBEV nucleotide sequences

Tick-borne encephalitis (TBE) virus is considered the medically most important arthropod-borne virus in Europe. Although TBE is endemic throughout central Europe, ticks and rodents determine its maintenance in small, difficult-to-asses, natural foci. We investigated the interrelation between the genetics of the main TBE virus (TBEV) vector tick (Ixodes ricinus), the most important reservoir host (Myodes glareolus, syn. Clethrionomys glareolus), and TBEV. Rodents and ticks were sampled on 15 sites within an exploratory study area, which has been screened regularly for TBEV occurrence in ticks for more than 10 years. On all 15 sites, ticks and bank voles were sampled, screened for TBEV presence via serology and RT-PCR, and genetically examined. Moreover, TBEV isolates derived from these analyses were sequenced. In long-term TBEV foci bank vole populations show extraordinary genetic constitutions, leading to a particular population structure, whereas ticks revealed a panmictic genetic stru..., Bank voles were sampled in 2019 using Sherman Traps. Ticks were sampled in 2019 using the flaggin method. TBEV isolates were dervied from positive samples. , , # Microsatellite data Myodes glareolus and Ixodes ricinus [https://doi.org/10.5061/dryad.cnp5hqcbn](https://doi.org/10.5061/dryad.cnp5hqcbn) The dataset consists of Microsatellite data of *Myodes glareolus* and *Ixodes ricinus* and Sanger sequenced Sequences of TBEV isolates for the article \"Interrelation of the spatial and genetic structure of tick-borne encephalitis virus, its reservoir host (Myodes glareolus), and its vector (Ixodes ricinus) in a natural focus area\". ## Description of the data and file structure The dataset \"`Microsatellite_data_Mg_Ir.xlsx`\" has the microsatellite data of *Myodes glareolus* on one sheet and of *Ixodes ricinus* on the other sheet. Empty cells represent no data, since this locus failed the amplification, or gel electrophoresis or the signal was unclear. Some column headers are blank, due to downstream software requirements (Microsatellite Toolkit). TBEV isolates where aligned and trimmed (`FSME_Seqs_all_aln_trim_6_W_Paper.fas`) and can be used fo...,蜱传脑炎（Tick-borne encephalitis, TBE）病毒被认为是欧洲医学领域最为重要的节肢动物传播病毒。尽管TBE在整个中欧地区均呈地方性流行态势，但蜱虫与啮齿类动物决定了该病毒在小型、难以评估的自然疫源地中的维持循环。本研究探讨了TBE病毒（TBEV）主要媒介蜱虫——蓖子硬蜱（Ixodes ricinus）、最重要的储存宿主——棕背䶄（Myodes glareolus，异名Clethrionomys glareolus）与TBEV三者之间的遗传关联。 研究于一个已连续10余年定期监测蜱虫中TBEV存在情况的探索性研究区域内的15个采样点开展采样工作，对啮齿类动物与蜱虫进行样本采集。在全部15个采样点中，均采集了蜱虫与棕背䶄样本，通过血清学与逆转录聚合酶链式反应（Reverse Transcription-Polymerase Chain Reaction, RT-PCR）筛查TBEV感染情况，并开展遗传学检测。此外，对本次分析中获得的TBEV分离株进行了测序。 在长期存在TBEV的疫源地中，棕背䶄种群展现出独特的遗传组成，形成了特殊的种群结构，而蜱虫则呈现泛交的遗传结构……2019年采用薛曼捕鼠器（Sherman Traps）采集棕背䶄样本。2019年采用拖旗法（flagging method）采集蜱虫样本。TBEV分离株源自阳性检测样本。 # 棕背䶄与蓖子硬蜱的微卫星数据 DOI: https://doi.org/10.5061/dryad.cnp5hqcbn 本数据集包含棕背䶄（Myodes glareolus）与蓖子硬蜱（Ixodes ricinus）的微卫星数据，以及为论文《自然疫源地中蜱传脑炎病毒、其储存宿主棕背䶄及其媒介蓖子硬蜱的空间与遗传结构的相互关联》所获得的TBEV分离株的桑格（Sanger）测序序列。 ## 数据与文件结构说明 数据集文件`Microsatellite_data_Mg_Ir.xlsx`包含两个工作表：分别存储棕背䶄的微卫星数据与蓖子硬蜱的微卫星数据。空单元格代表对应基因座未成功完成扩增、凝胶电泳失败或信号模糊。部分列标题因下游软件——微卫星工具包（Microsatellite Toolkit）的要求而留空。 已对TBEV分离株序列进行比对与修剪（文件名为`FSME_Seqs_all_aln_trim_6_W_Paper.fas`），可用于后续分析……