Human brain cell census for BA 44/45

Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive optical coherence tomography (PSOCT) enables registration of the distorted histological cell typing obtained with LSM to the MRI-based atlas coordinate system.