Streptococcus sanguinis SK36 - Obelisk-S.s sequencing for "Viroid-like colonists of human microbiomes"

Nucleotide sequencing of Streptococcus sanguinis SK36 harboring and lacking Obelisk-S.s grown under replete laboratory conditions.Namely, short read (Illumina, MiSeq v3 300 cycle kit) shotgun DNA (genomic DNA - NEB Monarch gDNA, T3010; Nextera XT, FC1311024; Unique Dual Indexes: DNA/RNA UD Indexes Set C, 20091648) and shotgun RNA (total RNA - RNAsnap; ribosome-depleted RNA - NEB bacteria rRNA depletion kit, E7860; or RNaseR-treated RNA - BioSearch, RNR07250; TaKaRa SMART-Seq total RNA mid input, 635049 - stranded, random hexamer, template switching; Unique Dual Indexes: Unique Dual Index Kit 96U Set B, 634457) of triplicate monocultures (aerobic, Brain Heart Infusion, 53286, 37 C, 12 hours inoculated at OD600 0.002) of Streptococcus sanguinis SK36 harboring (ObP1-A,B,C) and lacking (ObN1-A,B,C) Obelisk-S.s. Mock-inoculated, media-only controls (CTRL-A,B,C) also prepared. Additionally, longer read (Oxford Nanopore, PlasmidSaurus, "premium PCR", v14, R10.4.1) of Obelisk-S.s divergent RT-PCR (NEB LunaScript RT SuperMix, E3010, and NEB Ultra II Q5 Master Mix, M0544) amplicons (from ObP1-A,B,C total RNA with two different primer pairs: Disc-1-Fw, Disc-1-Rv, and Disc-2-Fw, Disc-2-Rv).sample UDI numberCTRL-A - total RNA U130CTRL-B - total RNA U131CTRL-C - total RNA U132ObP1-A - total RNA U133ObP1-B - total RNA U134ObP1-C - total RNA U135ObN1-A - total RNA U136ObN1-B - total RNA U137ObN1-C - total RNA U138CTRL-A - rRNAdep U139CTRL-B - rRNAdep U140CTRL-C - rRNAdep U141ObP1-A - rRNAdep U142ObP1-B - rRNAdep U143ObP1-C - rRNAdep U144ObN1-A - rRNAdep U145ObN1-B - rRNAdep U146ObN1-C - rRNAdep U147CTRL-A - RNaseR U148CTRL-B - RNaseR U149CTRL-C - RNaseR U150ObP1-A - RNaseR U151ObP1-B - RNaseR U152ObP1-C - RNaseR U153ObN1-A - RNaseR U154ObN1-B - RNaseR U155ObN1-C - RNaseR U156CTRL-A - DNA UDP0225CTRL-B - DNA UDP0226ObP1-A - DNA UDP0228ObP1-B - DNA UDP0229ObP1-C - DNA UDP0230ObN1-A - DNA UDP0231ObN1-B - DNA UDP0232ObN1-C - DNA UDP0233