Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs for three different transcription factors and a negative control

In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we generated miR-290-295 KO mutant cell lines, assessed their transcriptomes. We identified novel transcription factors (TF) as potential direct and functional targets of miR-290-295. To assess the role of these TFs, we rescued their expression in the miR-290-295 KO cell line using siPOOLs and profiled their transcriptomes. Transcriptomesof miR-290-295 KO E14 mESCs after siPOOL treatments for different Transcription Factors were generated in biological duplicates using QuantSeq kit.