Microarray analysis of Saccharomyces cerevisiae expressing the Hsp90 mutant hsc82-W296A. Saccharomyces cerevisiae

Altered mRNA levels of HBT1 were observed in S. cerevisiae cells expressing hsc82-W296A compared to WT HSC82. We conducted microarray analysis to determine the extent of other changes in that strain. Overall design: The yeast strain used contained chromosomal deletions of HSC82 and HSP82. WT HSC82 or hsc82-W296A was supplied on a plasmid so that it was the only Hsp90 present in the cell. Yeast were grown overnight in rich media at 30° and harvested during exponential growth phase. RNA was harvested from three cultures expressing WT Hsc82 and 3 cultures expressing the Hsp90 mutant..