Whole-genome mapping of RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac in human pre-B lymphoblasts, and B cell precursor leukemia (BCP-ALL)

Genome binding/occupancy profiling of human Runt-related transcription factor 1 (RUNX1), Far Upstream Binding Protein 1 (FUBP1) and histone marks by high throughput sequencing. RUNX1 and FUBP1 are two key transcriptional regulators implicated in hematopoiesis, from the maintenance of HSC to lineage-specific differentiation. We hypothesized that both proteins could play a joint role in transcription regulation of hematologic lineages. Here, we investigated the molecular proximity between RUNX1 and FUBP1 proteins on chromatin and their common target genes in pre-B lymphoblasts and B cell precursor leukemia (BCP-ALL). Altogether, our data show that RUNX1 and a subset of FUBP1 colocalize on chromatin in transcriptionally active/accessible chromatin regions mainly in promoters and enhancers regions. RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac genome-wide distributions were determined using ChIP-seq. Cells used in this study are human pre-B lymphoblast Nalm6 cell line and blasts from B-cell precursor acute lymphoblastic leukemia (BCP-ALL). ChIP samples and libraries were done by Sérandour A.A and Debaize L.