Molecular Pathology of adverse local tissue reaction caused by metal-on-metal implants

RNA-sequencing analysis of periacetabular synovial tissue collected from patients undergoing a revision total hip arthroplasty (rTHA) after failure of a Cobalt Chromium implant. Comparisons were made to periacetabular synovial tissue collected from patients undergoing primary THAs (pTHA). Results: Analysis of tissue biopsies by RNA-sequencing revealed that MoM patient samples exhibit significantly increased expression of immune response genes but decreased expression of genes related to extracellular matrix (ECM) remodeling. RNA was extracted from 3 pTHA and 3 rTHA samples and used for RNA-sequencing. Comparative analysis was performed using RPKM values obtained after processing of sequencing data.