Anti-oncogenic triterpenoids from Ganoderma lucidum activate circNF1-419 to regulate lipid metabolism and promote apoptosis in glioma

In order to confirm that L3 (G. lucidum triterpenoids fraction) could influence circRNA expression in glioma, U87 and L3-treated U87 cells were sequenced using an Illumina HiSeq 4000 sequencing system. There were 131 circRNAs identified as down-regulated and 131 circRNAs identified as up-regulated after the U87 cells were co-incubated with L3. Examination of circRNA sequending in 2 cell types (U87 and L3 treated U87 samples).