five

loss of DBP1 during meiosis in yeast

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE207267
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We used CRISPR/Cas9 to delete the DBP1 ORF in SK1 budding yeast cells. We then used ribosome profiling and mRNA-seq to observe gene expression profiles of wild-type and dbp1∆ cells during meiosis. Cells were grown as described in Powers et al., 2022. Samples were collected 6h post transfer to SPO media. Two biological replicates are included for each genotype.
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2023-01-05
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