RNAi components regulate the microparasitism in Trichoderma atroviride

Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained small RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata. The libraries were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC). Overall design: T. atroviride Wild type strain and dcr-2 mutant co-cultured with Alternaria alternata and collected at three stages: Before contact (BC), Contact (C) and After Contact (AC). The controls of T. atroviride were strains growing alone. Three replicates per condition. Total number of samples: 24