An ATP "Synthase" Derived from a Single Structural Domain of Bacterial Histidine Kinase
收藏中国科学院兰州化学物理研究所科学数据中心2026-01-09 更新2026-01-17 收录
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资源简介：
ATP (adenosine triphosphate) is a vital energy source for living organisms, and its biosynthesis and precise concentration regulation often depend on macromolecular machinery composed of protein complexes or complicated multidomain proteins. We have identified a single-domain protein HK853CA derived from bacterial histidine kinases (HK) that can catalyze ATP synthesis efficiently. Here, we explored the reaction mechanism and multiple factors that influence this catalysis through a combination of experimental techniques and molecular simulations. Moreover, we optimized its enzymatic activity and applied it as an ATP replenishment machinery to other ATP-dependent systems. Our results broaden the understanding of ATP biosynthesis and show that the single CA domain can be applied as a new biomolecular catalyst used for ATP supply.
提供机构：
中国科学院兰州化学物理研究所科学数据中心
创建时间：
2026-01-09
