Translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells in the presense or absence of PI3K inhibitor, 17β-hydroxy wortmannin

To see a possible effect of PI3K inhibition on a physiological warm temperature shift (WTS, 35°C to 38.5°C)-induced ribosomal accumulation on the Per2 minimal uORF, we performed Ribo-seq using dexamethasone-synchronized WTS-treated mouse embryonic fibroblast cells with or without 17β-hydroxy wortmannin (wortm) treatment at a circadain rising phase of Per2. We used Dex-synchronized WTS-treated MEF cells treated with or without wortm. WTS and wortm was applied 24 h after Dex synchronization (i.e., circadian rising phase of Per2). Two independent experiments were performed.