Total RNAseq of K562 cells with a doxycycline-inducible U2AF1S34F or U2AF1WT

Mutations in the splicing factor gene U2AF1 are relatively common in myelodysplastic neoplasms (MDS) and are associated with an inferior prognosis, but the molecular mechanisms underlying this are not fully elucidated. Circular RNAs (circRNAs) have been implicated in cancer, but it is unknown how mutations in splicing factors may impact on circRNA biogenesis. Here, we used RNA-sequencing to investigate the effects of U2AF1 mutations on circRNA expression in K562 cells with doxycycline-inducible U2AF1S34F or U2AF1WT. K562 cells with doxycycline-inducible expression of U2AF1WT or U2AF1S34F (generated previously, PMID 31011167) were subjected to total RNA sequencing (not PolyA selected), and circRNA reads were detected using the pipeline CIRI2.