PacBio sequence reads of amplicons to evaluate mosaicism from Cas9 mRNA or protein injected in bovine embryos alongside guide-RNAs targeting chromosomes 1, 17 or X
The validity of the LaacZ library was assessed by determining the number of embryos totally lacking SE labelling on a sample of 97 embryos. From this number (42) the number of embryos presenting N eve
Background: Cell-free nuclear DNA has been isolated from spent embryo culture medium. Whether this small amount of DNA can be amplified at the whole genome level and the concordance rate of kar