Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 31/12/2007 to 05/01/2008 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 31/12/2007 to 05/01/2008 on a trip from Hobart to Dumont D'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于星盘号科考船（RV L'Astrolabe，平台代码：FHZI），采集时段为2007年12月31日至2008年1月5日，航次为从霍巴特港前往迪蒙·迪尔维尔南极科考站的航程。光系统II（PSII）的最大光化学效率，又称PSII最大量子产额（Fv/Fm），是浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合机构捕获的光能用于光化学反应的概率。已有研究表明，Fv/Fm可对水体物理、化学性质的变化产生即时响应，常被用于诊断浮游植物的整体健康状况或生理活性。结合PSII吸收截面面积与叶绿素浓度数据，该参数可用于估算海洋初级生产力（Cheah等，2011，《深海研究》（Deep Sea Research））。本次实验通过船舶洁净海水管路连续抽取3米深度的海水。在暗适应状态下（暗适应时长为15分钟），以1分钟为间隔持续测定快速重复率（FRR）荧光产量；测定所用闪光序列包含两组：100次亚饱和微闪光（闪光时长1.1 μs，闪光间隔2.8 μs）与20次弛豫微闪光（闪光时长1.1 μs，闪光间隔51.6 μs）。