Analysis of transcriptomic changes with a special focus on processing sites in Synechocystis sp. PCC 6803 thermo-sensitive RNase E mutants before and after a 1 h heat shock

We compared transcriptomic changes, 5'-triphosphorylated (TSS) and 5'-monophosphorylated (PSS) RNA ends of a thermo-sensitive and a wild-typic RNase E mutant strain of the cyanobacterium Synechocystis sp. PCC6803 (rne(Ts) and rne(WT)) before and after a heat shock. Analysis of changing 5'-monophosphorylated ends revealed RNase E depedent processing sites on a transcriptome-wide level. Triplicates of RNA samples of strains rne(WT) and rne(Ts) grown at 30°C with and without heat treatment (39°C) were analysed