Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
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https://www.omicsdi.org/dataset/bioimages/S-JCBD-200901145
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sec6-4 cells expressing the immunoisolation bait Gap1GFPLTLM9 and the soluble secreted marker InvRFP from the inducible promoter Gals. Gap1GFPLTLM9 localizes to dot-like structures which is typical for the Golgi apparatus and endosomes in yeast. InvRFP is accumulating intracellularly at the restrictive temperature 37˚C. It is not secreted (shown also biochemically in Fig S3B).
创建时间:
2018-11-29



