Data from: Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

Plant protoplasts provide a starting material to induce pluripotent cell
masses in vitro competent for tissue regeneration. Dedifferentiation is
associated with large-scale chromatin reorganisation and massive
transcriptome reprogramming, characterized by stochastic gene expression.
How this cellular variability reflects on chromatin organisation in
individual cells and what are the factors influencing chromatin
transitions during culturing is largely unknown. High-throughput imaging
and a custom, supervised image analysis protocol extracting over 100
chromatin features unravelled a rapid, multiscale dynamics of chromatin
patterns which trajectory strongly depends on nutrients availability.
Decreased abundance in H1 (linker histones) is hallmark of chromatin
transitions. We measured a high heterogeneity of chromatin patterns
indicating an intrinsic entropy as hallmark of the initial cultures. We
further measured an entropy decline over time, and an antagonistic
influence by external and intrinsic factors, such as phytohormones and
epigenetic modifiers, respectively. Collectively, our study benchmarks an
approach to understand the variability and evolution of chromatin patterns
underlying plant cell reprogramming in vitro.