Protein-coding transcriptome of Murray-Darling rainbowfish (Melanotaenia fluviatilis) brain liver and gonads

The aim of the project was to generate a high-quality reference transcriptome for Murray-Darling rainbowfish to enable the design of genome-wide expression analysis strategies (such as microarrays) and to obtain protein-coding sequences for nuclear receptors and steroid biosynthetic enzymes involved in sexual development and reproduction. Assembled transcripts, predicted coding regions, deduced proteins and tentative descriptions are available in this collection.\nLineage: The project was supported internally through the Land and Water Capability Development Fund. Total RNA was isolated from brain, liver and gonads of adult male and female fish and mature messenger RNA sequenced using two lanes of Illumina HiSeq2000 in 100-cycle paired-end mode. Putative transcripts were prepared by de novo assembly and a non-redundant list of likely protein-coding regions was prepared and annotated by inference based on sequence homology.

本项目旨在为墨累-达令彩虹鱼构建高质量参考转录组，以支撑全基因组表达分析策略（如基因芯片）的设计，并获取参与性腺发育与生殖过程的核受体及类固醇生物合成酶的蛋白质编码序列。本数据集收录了组装得到的转录本、预测编码区、推导的蛋白质序列及初步注释信息。
数据生成流程：本项目由土地与水资源能力发展基金内部资助。研究人员从成年雌雄墨累-达令彩虹鱼的脑、肝脏及性腺中分离总RNA，随后采用Illumina HiSeq2000测序平台的两个测序通道，以100循环双端测序模式对成熟信使RNA进行测序。通过从头组装得到推定转录本，并基于序列同源性进行推断注释，最终生成非冗余的潜在蛋白质编码区列表。