Shp-deletion induces dissociation of steatosis and inflammation in NASH

Two months-old Shp flox/flox male mice were injected with either AAV8 expressing Cre recombinase driven by the thyroxine-binding globulin (Tbg) promoter (AAV8-Tbg-Cre) or control AAV8 (AAV8-Tbg-null) and fed chow or a diet enriched in high fat, cholesterol, and fructose (Research diet D09100301: 40 kcal% fat, 2% cholesterol, 20 kcal% fructose, hereafter referred to as HFCF diet) for 3 months. Liver RNA was isolated and submitted to RNA-seq. Analysis of hepatic gene expression from 3 WT mice on chow, 3 Shp Hep-/- mice on chow, 3 WT mice on HFCF diet, and 3 Shp Hep-/- mice on HFCF diet