TABLE 1 in Hermatomyces pyriformis sp. nov., a novel dematiaceous hyphomycete (Hermatomycetaceae, Pleosporales) associated with medicinal plants in Yunnan Province, China

TABLE 1. Genes, primers and amplification conditions of phylogenetic analyses in this study.
LocusPrimersOptimized PCR ProtocolsReferenceLSULR0R LR594 °C 3 min; 36 cycles of 94 °C 45 s, 55 °C 55 s, 72 °C 1 min; 10 min at 72 °C; 4 °C on holdVilgalys & Hester (1990)ITSITS5 ITS494 °C 3 min; 36 cycles of 94 °C 45 s, 55 °C 55 s, 72 °C 1 min; 10 min at 72 °C; 4 °C on holdWhite et al. (1990)tef1-αEF1-983F EF1-2218R94 °C 3 min; 38 cycles of 94 °C 45 s, 56 °C 55 s, 72 °C 1 min; 10 min at 72 °C; 4 °C on holdRehner & Buckley (2005)rpb2fRPB2-5F fRPB2-7cR95 °C 5 min; 40 cycles of 95 °C 15 s, 56 °C 50 s, 72 °C 2 min; 10 min at 72 °C; 4 °C on holdLiu et al. (1999)