Electrophysiology and Ca_imaging normalized data

The file contains the normalized electrophysiological (TEVC (two-electrode voltage clamp)) and calcium imaging (Ca-img) data for oocyte and Neuro2a cells responses, respectively. WT human α7 nAChR (nicotinic acetylcholine receptor) and mouse muscle nAChR were expressed in oocytes and Neuro2a cells. Besides, their mutants (at positions 117-119, 184, 185, 187 and 189 in α7 nAChR and at positions 153 and 190 in muscle nAChR) were heterologously expressed as well and their responses were estimated accordingly. Responses to different concentrations of acetylcholine (Ach) and epibatidine (Epi) were measured. For calcium imaging studies two calcium sensors (genetically encoded Case12 and low-molecular weight Fluo-4) were used. Calcium imaging analysis was carried out in the presence of a positive allosteric modulator PNU120596.本数据集包含分别对应卵母细胞与Neuro2a细胞响应的标准化电生理（双电极电压钳（two-electrode voltage clamp, TEVC））与钙成像（Ca-img）数据。野生型人源α7烟碱型乙酰胆碱受体（nicotinic acetylcholine receptor, α7 nAChR）与小鼠肌肉型烟碱型乙酰胆碱受体，分别在卵母细胞与Neuro2a细胞中完成异源表达。此外，两类受体的突变体（α7 nAChR的117-119、184、185、187及189位位点，肌肉型nAChR的153与190位位点）也被异源表达，并据此检测了其配体响应。研究测定了细胞对不同浓度乙酰胆碱（acetylcholine, Ach）与依匹啶定（epibatidine, Epi）的响应信号。钙成像实验中，共使用了两种钙传感器：基因编码型钙传感器Case12与低分子量钙染料Fluo-4。钙成像分析过程在阳性变构调节剂PNU120596的存在下开展。