Bathymetric adaptations of corals at Davies Reef, Great Barrier Reef: UV-B absorbing compounds

Corals were collected by scuba from depths between 1 and 45 m on the southeastern side of Davies Reef in October 1980 and February, July and December 1981 and the concentrations of mycosporine-like amino acids determined.Photosynthetic pigments and and mycosporine-like amino acids were extracted using 3 volumes of 20% tetrahydrofuran in methanol (v:v). Visible UV spectra were recorded and pigments were separated and quantified by high performance liquid chromatography. The UV-B band used in this study was 208-340 nm. To examine the relationship between the mycosporine-like amino acids of Acropora spp. and increasing depth.To determine whether Acropora spp. respond biochemically to the presence of UV-B light. Data analyses were part of a series of three papers addressing:1. Long-term growth responses (data not held by AIMS)2. Light saturation curves for photosynthesis and respiration (see separate metadata record)3. UV-B absorbing compoundsSee separate metadata records.

1980年10月、1981年2月、7月及12月，研究人员通过水肺潜水在戴维斯礁（Davies Reef）东南侧1至45米的水深范围内采集珊瑚样品，并测定了类菌孢素氨基酸（mycosporine-like amino acids）的含量。采用3倍体积的甲醇-20%四氢呋喃（体积比v:v）混合液提取光合色素与类菌孢素氨基酸；记录可见紫外光谱后，通过高效液相色谱（high performance liquid chromatography）分离并定量各类色素。本研究使用的紫外B（UV-B）波段范围为208~340 nm。
本研究旨在探究两个核心科学问题：一是鹿角珊瑚属（Acropora spp.）的类菌孢素氨基酸含量与水深增加之间的关联；二是鹿角珊瑚属生物是否会对紫外B光照产生生化响应。
本研究的数据分析属于三篇系列研究论文的内容之一，该系列论文涵盖以下主题：1. 长期生长响应相关研究（相关数据未由澳大利亚海洋科学研究所（Australian Institute of Marine Science，AIMS）留存）；2. 光合作用与呼吸作用的光饱和曲线研究（详见独立元数据记录）；3. 紫外B吸收化合物相关研究。相关细节请查阅独立元数据记录。