16S and 18S rDNA samples Metagenome. 16S and 18S rDNA samples

We used an experimental approach to simulate the effects of global warming predicted by different future scenarios on the prokaryotes (i.e. bacterioplankton) and eukaryotes (i.e. microeukaryotes) aquatic microbiota composition, and to evaluate the effects separately for each trophic level (autotrophic, heterotrophic and mixotrophic). The communities composition was obtained through high-throughput sequencing, using targeted markers for the 16S rDNA and 18S rDNA genes. To the best of our knowledge, our experiment is one of the first to simulate the effects of temperature increase using a metabarcoding approach for the prokaryotes and eukaryotes tropical freshwater microbiota . Our results contribute to predict how different biological levels and trophic groups of the aquatic microbiota respond to global warming. This approach is relevant because warming leads to changes in the communities composition and affects ecosystem processes essential to the aquatic environment.