SRE4 Effects of hydrocarbons on Antarctic Marine Sediment Ecosystems - SRE4_Meiofauna

Sediment Recruitment Experiment 4 (SRE4) was a large, long term (5 year) field experiment run at Casey Station (from 2001 to 2006) testing the effects of 4 different hydrocarbons on marine sediment ecosystems. Four different types of hydrocarbons were individually mixed with defaunated marine sediments and deployed in trays on the seabed at O'Brien Bay-1. Trays were collected after deployment periods of 5 weeks, 56 weeks (one year), 62 weeks, 2 years and 5 years. In addition there was a bioturbation treatment using the burrowing urchin Abatus (at 56 weeks only). Samples were collected from 4 replicate trays of each treatment at each sampling time. Analyses were done of sediment hydrocarbon chemistry, microbial communities, meiofaunal communities, macrofaunal communities and diatom communities. The hydrocarbon treatments were: a synthetic Mobil lubricating oil; the same Mobil lubricating oil after 125 hours use in a vehicle engine; a Fuchs synthetic lubricating oil marketed as highly biodegradable; and Special Antarctic Blend diesel fuel (SAB). A control uncontaminated sediment treatment was used for comparison.
A randomised block design was used with a tray of each treatment in 24 blocks (5 trays per block). Four blocks were sampled at each time (4 trays of each treatment).
Meiofauna
Two replicate cores were taken from each tray at each time.
Copepods were identified to family. Nematodes were identified to genus.
Data is from one, two and five years of the experiment.
At one year there is only one replicate per tray for copepod data (a total of 4 replicates per treatment).

沉积物招募实验4（Sediment Recruitment Experiment 4，简称SRE4）是一项为期5年的大型长期野外实验，于2001年至2006年在南极凯西站开展，旨在探究4种烃类物质对海洋沉积物生态系统的影响。实验将四种不同烃类分别与脱除底栖生物的海洋沉积物均匀混合，随后将混合物装填至实验托盘，并布设至奥布莱恩湾-1号站位的海床区域。分别于布设后5周、56周（即1年）、62周、2年及5年时回收实验托盘。此外还设置了一项生物扰动处理组，使用穴居海胆Abatus开展实验，该处理仅在56周采样节点进行。每个处理组在每个采样时间点均采集4个重复托盘的样本，分析内容涵盖沉积物烃类化学组成、微生物群落、小型底栖生物群落、大型底栖生物群落以及硅藻群落。
本次实验的烃类处理组具体包括：合成型美孚润滑油；经车辆发动机运转125小时后的同款美孚润滑油；一款以高生物降解性为宣传卖点的福斯（Fuchs）合成润滑油；以及特种南极混合柴油（Special Antarctic Blend diesel fuel，简称SAB）。同时设置未受污染的沉积物对照组以作为参照。
本实验采用随机区组设计，每个区组内包含各处理组对应的1个托盘，共计24个区组（每个区组含5个托盘）。每个采样时间点采集4个区组的样本，即每个处理组对应4个托盘样本。
小型底栖生物（Meiofauna）相关样本采集规范：每个托盘在每个采样时间点均采集2个重复岩心样本。其中桡足类（Copepods）鉴定至科分类阶元，线虫（Nematodes）鉴定至属分类阶元。
本实验公开的数据涵盖1年、2年及5年三个采样时间节点。在1年采样节点中，桡足类数据仅保留每个托盘的1个重复样本，每个处理组总计4个重复样本。