Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Impact of Capillary Length on Sequence Quality
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https://www.ncbi.nlm.nih.gov/sra/SRP295728
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The impact of sequencing conditions on sequence quality was evaluated by sequencing a variety of samples, including HMP even Mock community and clinical samples. The following PCR parameters were tested in addition to the original protocol: lower cycle number (20x), and longer extension time (10 min.)
创建时间:
2020-12-03



