The relative number of mispositioned cells (GATA6 cells in epiblast and NANOG cells in endoderm) at the end of simulation.

The simulation starts from 12 to 14 ICM cells half of which are NANOG and another half GATA6. The cells are allowed to take three rounds of divisions and then their position (endoderm or epiblast) and gene expression (GATA6 or NANOG) is recorded. Number of mispositioned GATA6 (NANOG) cells relative to the total number of epiblast (endoderm) cells is presented for simulations of different separation mechanisms. Numbers for each model are averages of 6 simulations. In random model (first column) cells are labeled as GATA6 and NANOG but there is no difference in their adhesion strengths or directional movement. The differential adhesion model (second column) assume that GATA6 cells have slightly lower self-adhesion than NANOG cells. Cross-adhesion between both types is small as well. The directional movement model without differential adhesion (third column) features constant force drawing the GATA6 cells towards endoderm position. The lowest number of misplaced cells is obtained by combination of differential adhesion and directional signal (fourth column).