Transcriptomic alterations in stably overexpressing (OE) clones of solute carriers SLC22A10 and SLC22A15 in PANC-1 (human pancreatic cancer cell line)

We conducted RNA-seq analysis of vector (control), SLC22A10_OE and SLC22A15_OE clones to identify any SLC22-specific oncogenic alterations associated with pancreatic cancer progression. Our findings demonstrate substantial activation of key regulators of epithelial-mesenchymal transition (EMT), cancer cell invasion, and drug resistance such as receptor tyrosine kinase like orphan receptor 1 (ROR1) and aldehyde dehydrogenase 1A1 (ALDH1A1) along with enrichment of interferon alpha and gamma signaling pathways in SLC22A10_ and SLC22A15_OE clones compared to the vector clone. mRNA profiling of vector versus SLC22A10_OE and vector versus SLC22A15_OE clones