Studied genes, DNA sequences for forward and reverse primers (5΄-3΄), GenBank accession numbers, the PCR product length and approximate amplification efficiency.

aADIPOQ, adiponectin; ADIPOR1, adiponectin receptor 1; ADIPOR2, adiponectin receptor 2; INSR, insulin receptor; LEP, leptin; MCP-1, monocyte chemoattractant protein-1; MRPL39, mitochondrial ribosomal protein L39; RBP4, retinol binding protein 4; SCD, stearoyl CoA desaturase; TNF, tumour necrosis factor-alpha.bFor, forward; Rev, reverse; exon-exon junctions are underlined.cGenBank accession numbers are from the databases of the National Center for Biotechnology Information; all sequences were derived from horse (Equus caballus).dE, approximate amplification efficiency for each gene calculated from amplification curves as described by Selim et al. [30].Studied genes, DNA sequences for forward and reverse primers (5΄-3΄), GenBank accession numbers, the PCR product length and approximate amplification efficiency.