File S1 - Functional Characterization of the Poplar R2R3-MYB Transcription Factor PtoMYB216 Involved in the Regulation of Lignin Biosynthesis during Wood Formation

Contains: Table S1 List of primers used for qRT-PCR. Figure S1. Photographs of PtoMYB216 overexpressing Populus tomentosa Carr. after four weeks of growth. No phenotypic or growth differences were observed between PtoMYB216 overexpressing and control plants under the same growth conditions. Figure S2. PCR analysis of transgenic poplar plants. Genomic DNAs were isolated from hygromycin-resistant plants transformed with the 35S:PtoMYB216 vector. PCR amplification using primers specific for the production of a 562-bp PtoMYB216 fragment. M, D2000 DNA Ladder; WT, non-transgenic plants; P, corresponding plasmid DNA (positive control); Lanes 1–10, independent transgenic lines. Numbers on the left indicate DNA marker sizes in base pairs. (DOC)