Figure_1.jpg

(A) Effect of 1,25(OH)2D3 on metabolic activity. Three LOP cell populations (LOP 1, LOP 2, and LOP 3) were cultured in standard medium (CONTROL), osteogenic medium (OM), and OM + 1nM of 1,25(OH)2D3 (OM + VD), and the MTT assay for assessing cell metabolism and viability was performed at 1, 3, 7 and 10 days. The control represents 100% of viability. (B) VDR expression after 1,25(OH)2D3 treatment. Real-time PCR analysis after treatment confirmed that all three populations express mRNAs for VDR. Experiments were performed in triplicate three times, with comparable results obtained on each occasion. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences are indicated by different lowercase letters, and intragroup statistical significant differences are indicated by different uppercase letters. The letter ‘X' represents a difference with respect to day 1. The letter 'Y' represents a difference with respect to day 3. The letter 'Z' represents a difference with respect to day 7(P < 0.05).