Transcriptomic profile of circulating CD8 T cells in donors with ZIKV infection

The pandemic rise of Zika virus (ZIKV) has recently commanded the attention of the general public and medical research community alike . The characterization of exact molecular components of immune response associated may help design more effective therapeutic interventions. In this study, we aimed to characterize the immune signature of ZK-specific CD8 T cells after stimulation with ZK specific peptides comparing it with a generic stimulation with anti-CD3/CD28. Transcriptomic profiling using RNA sequencing was performed on CD8 T cells producing IFNg in comparison with non IFNg producing cells isolated from individuals with Zika infections from endemic areas of Puerto Rico and US travellers. Overall design: RNA-sequencing of sorted CD8 T cells from cryopreserved PBMC of 16 subjects with ZIKV infection.